不同移栽期烤烟化学成分与香味成分的相关性和主成分分析

为了探讨气候资源配置差异,解决生产后期不利因素制约烟叶成熟、影响烟叶品质的问题,通过适度提前移栽并对不同移栽期烤后烟叶化学成分、香味成分、外观及评吸等54项指标间相关性及主成分分析,发现甜度、糠醛、糠醇、巨豆三烯酮1、巨豆三烯酮4和叶片生长期有效积温均值等6个指标与其余指标间表现出高度的相关性。3月10日移栽烟叶的上部叶色度最好,总氮较高且总糖最低,同时中部叶的甜度最高且苯乙醇含量最低,能较好地符合皖南优质烟质量评价指标体系,生产上可以探索适度提前移栽获得较好中部叶和上部叶的配套栽培措施。     

高校校园网络流量分流控制与优化

本文以甘肃省陇东学院校园网为研究对象,针对目前高校校园网络普遍存在的网络带宽使用不合理,链路拥塞等问题,提出部署专业流量控制设备,实现流量分流,优化应用策略,提高网络安全防护等措施。实践证明,该方案的使用使校园网络带宽得到了明显提升,教学、科研和管理等关键网络应用得到了有力保障。     

南京地区西瓜秋延迟栽培技术研究

为延长西瓜供应时间,实现反季节上市,提高大棚利用率,研究了南京地区西瓜秋延迟栽培技术。结果表明:‘早佳8424’和‘小兰’为最适宜南京地区秋延迟栽培的中型和小型西瓜品种;采用地爬式栽培,西瓜单果重,商品性好,投资较小;采用吊蔓栽培,栽培密度大,西瓜亩产高;种苗8月上旬定植,生长期避开高温,利于高产增收。     

适应机械化收获的田间油菜植株特性研究

油菜机械化收获过程出现的高损失率制约了油菜生产的发展,单纯从农机方面进行研究难以解决。该文从农机农艺相结合出发,对田间油菜植株特性进行测试研究。测试大田植株整体表现特性及不同密度下植株茎秆物理特性的田间表现,并在不同成熟度和催熟剂喷施情形下对油菜角果拉断力进行测试,获得了田间植株特性基础数据。结果表明适宜的种植密度有利于机械化收获作业,催熟剂的喷施对机收作业性能影响不大。研究获得了直播油菜的田间植株表现特征,同时为油菜机收配套农艺要求提供理论研究基础和参考依据。     

低温发酵乳品质特性研究

以青藏高原传统发酵牦牛乳中分离的保加利亚乳杆菌（编号为CGMCC2603）和酸乳球菌（编号为CGMCC1.3920）为研究对象，研究菌株低温发酵酸乳的感官特性、后酸化能力、粘度及双乙酰含量变化。结果表明试验菌株在25℃发酵的酸乳凝乳状态较好，冷藏14d后酸度分别提高29．2°T和8．5°T，后酸化较弱。冷藏期间粘度变化与发酵温度没有直接关系。低温有利于双乙酰的积累，双乙酰含量最高分别为12．04ug／mL和9．78ug／mL。本研究时开发低温发酵剂具有重要意义。     

Differential Expression Analysis of MUC4 and MUC13 Genes Between Resistant and Sensitive Weaned Meishan Piglets to ETEC F18

MUC4 and MUC13 genes as important candidate genes for enterotoxigenic Escherichia coil (ETEC) F4 resistance,may play an important role in the process of against ETEC F18 infection in weaned piglets. In this study,ETEC F18-resistant and -sensitive weaned Meishan piglets were used,and the expression levels of MUC4 and MUC13 genes in 11 tissues (heart,liver,spleen,lung,kidney,stomach,muscle,thymus,lymph nodes,duodenum and jejunum) were determined by quantitative Real-time PCR. The results showed that MUC4 and MUC13 genes were broadly expressed with different expression levels in all the 11 tissues. In the thymus and lymph tissues,the expression of MUC4 gene in resistant piglets was significantly higher than that in sensitive piglets (P<0.05);In the lung tissue,theMUC13 gene expression level in resistant individuals was significantly higher than that in sensitive individuals (P<0.05),and in the intestinal tissues of duodenum and jejunum, the expression level of MUC13 gene was relatively higher in resistant individuals. Thus we speculated that the high expression of MUC4 gene in immune tissues and MUC13 gene in intestinal tissues might improve the immune ability of piglets,protect and lubricate the intestinal tract, and resist ETEC F18 infection.     

TRIZ创新方法在旱地高速移栽技术研究中的应用

结合旱地高速移栽技术研究课题,针对改善移栽机苗盘输送准确度、提高取苗装置取苗稳定性等关键技术难点,基于发明问题解决理论(TRIZ)的思想和方法,提出苗盘精准输送方案和取、投苗装置结构优化方案,并将新方案运用于2ZBJ-4型悬挂式旱地移栽机样机试制且进行了试验。试验表明:苗盘输送准确度高,运行稳定,取、投苗可靠,取苗效率7 000株/h·单元以上,最高超过10 000株/h·单元,移栽机作业效率60~90株/min·行,平均漏栽率低于3%,栽植合格率大于9 2%,性能优良。通过TRIZ理论在旱地高速移栽机技术研究中的运用,验证了创新方法流程在农业科技项目中应用切实可行。     

DXDK-40型自动包装机成型器及热压封头改进设计

在包装工程实验教学中,发现DXDL型立式全自动包装机存在供膜走偏、热封皱褶甚至密封不好等问题。本文结合机械设计的相关理论,探索出影响薄膜供给和材料成型缺陷的根本原因是热压封头的封合压力混乱以及成型器的外部曲线存在拐点突变。针对上述问题,重点研究热压封头和象鼻成型器的改进方案及设计方法,从理论上对该立式包装机的封合和成型进行创新性设计。     

Amplification,Sequence Analysis and Tissue Expression Detection of FUT2 Gene Coding Regions of Dongchuan Pigs

Porcine alpha (1,2) fucosyltransferase (FUT2) gene was importance in glycosphingolipid biosynthesis-globo series, potentially played a regulatory role during Escherichia coli (E. coli) F18 infection process in weaned piglets. In order to explore sequence structure of porcine FUT2 gene and its biological function, this test amplified FUT2 gene CDS sequence of Dongchuan pigs by PCR, forecasted and analyzed the protein sequences and functional regions of FUT2 gene, its expression level was detected in 11 tissues of 8 Dongchuan weaned piglets in 35 days old at the meantime. The results showed that the CDS sequence of FUT2 gene was 1 023 bp, which encoded 340 amino acids. FUT2 protein was fat-soluble hydrophilic protein, which the structure was not stable, including a transmembrane helix structure, but without signal peptide that suggested the FUT2 protein was a membrane protein;FUT2 protein included 2 N-glycosylation sites (No. 185 and No. 305 amino acids), without O-glycosylation sites, there were 14 potential phosphorylation sites, included 6 Ser, 2 Thr and 6 Tyr, analyzing the functional regions found that the FUT2 protein had a superfamily of conserved domains:FUT1-FUT2-like (58-319 amino acids). The phylogenetic tree result showed that the relative relationship between swine and cattle was relatively close, but was distant from chimpanzee, human, mouse and rat. FUT2 gene was expressed in all 11 tissues of Dongchuan weaned piglets, there were higher expression in digestive tract and immune tissues. The present results suggested that FUT2 gene might play a role to resistance to E. coli F18 in weaned piglets, and might indirectly against E. coli F18 through the synthesis of fucosyltransferase.     

多年施用氮肥对水曲柳人工林内生菌根真菌侵染及其根尖形态的影响

以水曲柳人工林1级根为研究对象,通过施氮肥处理,对不同季节和不同土壤深度根系取样,研究施用氮肥对根尖内生菌根真菌侵染率和形态与土壤N有效性、季节和土壤深度的关系.结果表明:1)施N肥处理导致水曲柳人工林1级根的菌根真菌侵染率显著降低11%,季节和土壤深度对1级根的侵染率影响显著,其中春季＞夏季＞秋季,土壤表层高于亚表层;2)施N肥导致1级根长度显著降低,季节和处理之间的交互作用影响1级根的直径和根长,季节、土层和处理3者的交互作用影响根长和比根长;3)与未侵染菌根真菌的1级根形态相比,菌根真菌侵染导致1级根平均直径未发生变化,长度略有降低,而比根长则显著减小.这种根系形态变化规律在不同处理、不同季节和不同土壤深度上表现一致.